Candidatus liberibacter solanacearum lso has emerged as a serious threat worldwide. The prefix candidatus is used in a unique trinomial name system, which indicates that the bacterium is unculturable. First report of candidatus liberibacter solanacearum associated with the psyllid bactericera trigonica hodkinson on. Bacteria, proteobacteria, alphaproteobacteria, rhizobiales, rhizobiaceae, candidatus liberibacter common names. Bactericera cockerelli is a vector of candidatus liberibacter solanacearum, the pathogen associated with zebra chip disease of potato solanales. The draft wholegenome sequence of candidatus liberibacter solanacearum strain r1, isolated from and maintained in tomato plants in california, is reported. Candidatus liberibacter solanacearum haplotypes d and e in. Introduction the symptoms on peppers include stunted growth, and. Browncolonization and intrusive invasion of potato psyllid by candidatus liberibacter solanacearum. Bactericera cockerelli and candidatus liberibacter solanacearum. Pdf candidatus liberibacter solanacearum, associated with.
This pest data sheet provides basic scientific information about candidatus liberibacter solanacearum and the damage it can cause. Symptoms including leaf curling, yellowing, bronze and purplish discoloration, stunting of plants and roots, and proliferation of secondary roots, affected 20 to 40% of the carrots in some plots. Liberibacter solanacearum infection on ph a and oxygen tension b in potato psyllid midgut. Candidatus liberibacter solanacearum is a bacterial plant pathogen that is exotic to australia. Pdf first report of candidatus liberibacter solanacearum. Apr 30, 2018 vegetative disorders similar to those associated with the presence of candidatus liberibacter solanacearum calsol were observed in carrot plants in kairouan, tunisia from 2014 to 2016. Description of candidatus liberibacter solanacearum candidatus liberibacter solanacearum so. Candidatus liberibacter solanacearum is found in all states west of the rocky mountains, kansas and in texas. This document is pp320, one of a series of the plant pathology department, ufifas extension. First report of candidatus liberibacter solanacearum in. Candidatus liberibacter solanacearumpositive psyllidspecimenstotal of analyzed specimens total captured b the three positive specimens belong to bactericera sp. Candidatus liberibacter solanacearum, associated with plants in the family solanaceae. Pm100228 eppo a1 and a2 lists of pests recommended for regulation as quarantine pests 2019.
The fastidious nature of these pathogens has hindered the study. The complete genome sequence of candidatus liberibacter solanacearum, the bacterium associated with potato zebra chip disease hong lin1, binghai lou1,2. Pathogenfree potato plants were individually exposed to. Candidatus liberibacter solanacearum calsol is a phloemlimited bacterium that is the causal agent of the potato disease zebra chip. It describes how the plant health service for england will respond if the solanaceous haplotypes of candidatus liberibacter solanacearum lso andor their psyllid vector bactericera cockerelli, which together constitute the zebra chip complex, are discovered on. Documents about candidatus liberibacter solanacearum libeps number title download.
Pdf mycorrhization mitigates disease caused by candidatus. Sep, 2017 the occurrence of candidatus liberibacter solanacearum lso was determined in commercial and wild species of the family apiaceae, obtained from three seed collections held in the united kingdom. Jun 01, 2011 candidatus liberibacter solanacearum is the putative causal agent of zebra chip disease of potato, solanum tuberosum l. We report here the complete genome sequence of candidatus liberibacter africanus strain ptsapsy. Haplotypes a and b are present in the americas andor new zealand, where they are vectored to solanaceous plants by the potato psyllid, bactericera. No phytoplasmas were detected in seedlings with symptoms using a real. Nissinen6, minna pirhonen1 1 department of agricultural sciences, fi00014 university of helsinki, helsinki, finland, 2 herne genomik. Understanding the process of transmission of clso by psyllids is fundamental to devise sustainable management strategies.
Detection of candidatus liberibacter solanacearum in the. Although only small quantities of each seed accession was tested, a high proportion of commercial. Report of a pest risk analysis for candidatus liberibacter solanacearum in. A liberibacter isolate nz082226 was detected in a symptomatic tomato plant and subsequently in five other members of the family solanaceae. Candidatus liberibacter solanacearum haplotypes d and e. Pest information candidatus liberibacter solanacearum is a phloemlimited, gramnegative, unculturable bacterium that is associated with several emerging diseases. Zc has been associated with a previously undescribed species of liberibacter, tentatively named candidatus liberibacter solanacearum, also known as ca.
It is considered very invasive due to its ability to be transported primarily in infective psyllids munyaneza et al. The potato psyllid, bactericera cockerelli sulc hemiptera. Liberibacter solanacearum lso andor their psyllid vector bactericera. Candidatus liberibacter solanacearum was first proposed as the name for the putative causal agent of zebra chip in potato solanum tuberosum l. Candidatus liberibacter solanacearum lso is a phloemlimited, gramnegative, unculturable bacterium that is primarily spread by psyllid insect vectors. Insects free fulltext a transcriptomics approach reveals.
Candidatus liberibacter solanacearum agriculture and food. The national regulatory control system pm 925 1 bactericera cockerelli and candidatus liberibacter solanacearum provides guidance on excluding b. Detection of candidatus liberibacter solanacearum in carrot seed. Pm 7143 1 candidatus liberibacter solanacearum 2020 eppo. Outbreaks of zebra chip disease candidatus liberibacter solanacearum andor its psyllid vector bactericera cockerelli in potato crops fig. Temperature has been shown to have a significant effect on development of liberibacter species associated with citrus huanglongbing disease. Development of a pcr assay for the rapid detection and. Candidatus liberibacter solanacearum associated with the. This standard describes a diagnostic protocol for candidatus liberibacter solanacearum, and for its detection in the psyllid vectors bactericera cockerelli, trioza apicalis and bactericera trigonica. Candidatus liberibacter solanacearum is the putative causal agent of zebra chip disease of potato, solanum tuberosum l.
Vahling4 1united states department of agricultureagricultural research service, cdpg, san joaquin valley. Jan 17, 2014 accurate detection of the three species associated with hlb disease, candidatus liberibacter asiaticus, candidatus liberibacter africanus and candidatus liberibacter americanus, is essential for the preventive control of the disease. These bacterial species have been associated with serious diseases of. Distribution of candidatus liberibacter species in eastern.
This document is pp320, one of a series of the plant pathology department, ufifas extensi. Candidatus liberibacter asiaticus accumulates inside. Candidatus liberibacter solanacearum and its vector bactericera cockerelli. Although only small quantities of each seed accession was tested, a high proportion of commercial carrot seed lots. The european and mediterranean region is currently threatened with several new.
Candidatus liberibacter solanacearum libepsoverview. Candidatus liberibacter solanacearum, associated with. Effects of temperature on candidatus liberibacter solanacearum and zebra chip potato disease. Article pdf available in plant disease 1011 september 2016 with 150 reads. Research article genomic sequence of candidatus liberibacter solanacearum haplotype c and its comparison with haplotype a and b genomes jinhui wang1, minna haapalainen1, thomas schott2, sarah m. Pm 7143 1 candidatus liberibacter solanacearum 2020. Effects of temperature on candidatus liberibacter solanacearum and zebra chip potato disease symptom development. Candidatus liberibacter solanacearum 20 eppo bulletin. You may reuse this information not including logos free of charge in any format or medium. General features of the candidatus liberibacter solanacearum genome and comparison to ca. Candidatus liberibacter solanacearum is an exotic pest to australia. Genomic sequence of candidatus liberibacter solanacearum.
Pdf a liberibacter isolate nz082226 was detected in a symptomatic tomato plant and subsequently in five other members of the. Rapid pest risk analysis for candidatus liberibacter. Triozidae is a serious pest of potato and other solanaceous crops. Detection, distribution, and genetic variability of. Candidatus liberibacter solanacearum haplotype e was identified in the seeds and seedlings of cv. Mar 14, 2020 1 introduction candidatus liberibacter solanacearum is a gram. Epidemiology and diagnosis of potato phytoplasmas and candidatus liberibacter solanacearum and their contribution to risk management in potato and other crops phylib edinburgh, 12 october, 2014. The infection of its insect vector by bacterial plant.
The specificity and sensitivity of polymerase chain reaction pcr primers developed for candidatus liberibacter solanacearum and candidatus liberibacter psyllaurous were evaluated in. Much work has been done on the association of liberibacter with the potato psyllid and several laboratories currently use polymerase chain reaction pcr to test for the bacterium. The following is the supplementary data to this article. Detection of the liberibacters is based on pcr amplification of their 16s rrna gene with specific primers. First report of candidatus liberibacter solanacearum in carrot in france. Candidatus liberibacter solanacearum revision history. It is restricted to the host plants phloem and psyllid vectors hemolymph, alternating its life cycle between host plants and insect vectors. General information about candidatus liberibacter solanacearum libeps name authority.
Candidatus liberibacter solanacearum, associated with plants. These involve the planting of disease free material, removal of infected. The accessions dated from the 1970s and the seed had been sourced worldwide. Complete genome sequence of candidatus liberibacter. Earlier reports refer to the pathogen as liberibacter solanacearum or candidatus. Gottwald tr 2010 current epidemiological understanding of citrus.
Candidatus liberibacter is a genus of gramnegative bacteria in the rhizobiaceae family. The disease was first seen in potato in mexico and parts of central america in the 1990s and then was spread to southern states of the us by its vector, the tomatopotato psyllid bactericera cockerelli and also into new zealand possibly with. Dp 21 diagnostic protocols for regulated pests dp 212 international plant protection convention 1. Candidatus liberibacter solanacearum plant health portal. Candidatus liberibacter solanacerum clso, transmitted by bactericera trigonica in a persistent and propagative mode causes carrot yellows disease, inflicting hefty economic losses. Rapid pest risk analysis for candidatus liberibacter solanacearum. Candidatus liberibacter solanacearum suggest longstanding separation. Phylogenetic analyses of the 16s rrna gene sequence, the deduced amino acid sequence of the rplj gene and a partial nucleotide sequence of the. Host free biofilm culture of candidatus liberibacter asiaticus, the bacterium associated with huanglongbing. Lso is one of six candidatus liberibacter species liefting et al. Hostfree biofilm culture of candidatus liberibacter. Candidatus liberibacter solanacearum titer over time in. Candidatus liberibacter solanacearum libepsdocuments. Haplotypes a and b are present in the americas andor new zealand, where they are vectored to solanaceous plants by the potato psyllid, bactericera cockerelli sulc hemiptera.
Candidatus liberibacter bacterial species are phloemlimited, gramnegative, unculturable bacteria vectored by psyllids 1,2,3. Detection of candidatus liberibacter solanacearum in. This is the first liberibacter species described from a nonrutaceous host. Transmission tests of candidatus liberibacter solanacearum. Vegetative disorders similar to those associated with the presence of candidatus liberibacter solanacearum calsol were observed in carrot plants in kairouan, tunisia from 2014 to 2016. Development of primers for improved pcr detection of the potato zebra chip pathogen, candidatus liberibacter solanacearum. Huanglongbing hlb disease is seriously threatening andor damaging the citrus industry worldwide. Marianne loiseau 1, soraya garnier2, violaine boirin3, maryse merieau4, anne leguay1, isabelle renaudin 1, jeanphilippe renvoise 2, pascal gentit 1, francoise. The disease was first reported in the united states in 2004.
A novel haplotype of candidatus liberibacter solanacearum found. Candidatus liberibacter solanacearum the cause of zebra chip disease in potato. Persistent transmission involves critical steps of adhesion, cell invasion, and replication. Civerolo,1 chuanwu chen2, yongping duan4, lijuan zhou5, cheryl m. Candidatus liberibacter solanacearum infects pepper plants. The complete genome sequence of candidatus liberibacter. Transmission of candidatus liberibacter solanacearum in. Plants free fulltext mycorrhization mitigates disease caused by. Genomic sequence of candidatus liberibacter solanacearum haplotype c and its comparison with haplotype a and b genomes jinhui wang1, minna haapalainen1, thomas schott2, sarah m. In this version an internal amplification control is added to the seqpcr background in ishivegs view there is a lack of evidence for seed transmission of candidatus liberibacter solanacearum ca. After 150 days, symptoms of proliferation were observed in 12% of seedlings of cv.
A survey of candidatus liberibacter solanacearum in. An emerging pathogen infecting potato and tomato1 binoy babu, mathews l. The occurrence of candidatus liberibacter solanacearum lso was determined in commercial and wild species of the family apiaceae, obtained from three seed collections held in the united kingdom. Accurate detection of the three species associated with hlb disease, candidatus liberibacter asiaticus, candidatus liberibacter africanus and candidatus liberibacter americanus, is essential for the preventive control of the disease real. Disease caused by the bacterial pathogen candidatus liberibacter solanacearum lso represents a serious threat to solanaceous crop production. The term candidatus indicates that it has not proved possible to maintain this bacterium in culture. Candidatus liberibacter solanacearumhaplotype c and identi. This pest specific response plan has been prepared by the defra risk and policy team. Inability to culture the phloemrestricted alphaproteobacterium candidatus liberibacter asiaticus ca. It can affect both solanaceous and apiaceous crops.